Anthony De Tomaso
Awards / Honors
- Ellison Foundation New Scholars in Aging Award
Dr. De Tomaso received his BS degree in Biology from Stanford University and his PhD in Cellular and Molecular Biology at Washington University School of Medicine in St. Louis. His doctoral thesis focused on understanding the mechanisms of multi-subunit protein assembly and targeting, using the rodent Na,K-ATPase. Following completion of his PhD, Dr. De Tomaso was a NIH fellow in the laboratory of Irv Weissman at Stanford University, where he worked on both delineating the molecular mechanisms which underlie allorecognition in the primitive chordate, Botryllus schlosseri, as well as understanding the cellular and molecular basis of regeneration in this organism.
Research in our lab is based around the phenomenon of self/non-self recognition (allorecognition) in a primitive chordate organism, Botryllus schlosseri. This allorecognition reaction links a number of disparate fields, including immunology, stem cell, developmental, and evolutionary biology, and also has ecological consequences. Several unique aspects of the Botryllus life history make it a novel, experimentally accessible model organism to ask pertinent questions in these distinct disciplines.
Botryllus individuals undergo a natural transplantation reaction when they come into contact with other members of the same species. Using a combination of genetic, genomic and cell biological approaches, we are focused on understanding the molecular mechanisms which underlie this reaction, which is similar to the MHC-based allorecognition found in the vertebrates, and functionally resembles the way in which bone marrow transplants are recognized and rejected in mice and humans. This allorecognition system exists to prevent the transplant of blood-borne pluripotent stem cells, which once transferred can parasitize other genotypes, replacing their somatic and/or germline tissues. Parasitic abilities are genetically determined and autonomous to the cells themselves, and we are studying the mechanisms which underlie these properties. Transplanted stem cells are able to parasitize other individuals because the adult body plan of Botryllus is colonial, and has an asexual reproductive pathway that can regenerate adult individuals through an independent developmental program lacking any embryonic stages-the only chordate which can do so. The developmental pathways which control the expansion, differentiation, and patterning of asexual regeneration are unknown, and we are studying this process using a variety of techniques. Botryllus occupies a key position in vertebrate evolution, and these studies may provide insights into the origins of many chordate innovations.
Langenbacher, A.D., and De Tomaso, A.W. (2016) Temporally and spatially dynamic germ cell niches in Botryllus schlosseri revealed by expression of a TGF-beta family ligand and vasa. EvoDevo 7:9
Kassmer, S.H., Rodriguez, D., and De Tomaso A.W. (2016) Colonial ascidians as model organisms for the study of germ cells, fertility, whole body regeneration, vascular biology and aging. Curr Op Genet Dev 39:101-106.
Rodriguez, D., Kassmer, S.H. and De Tomaso, A.W. (2016) Gonad development and hermarphodism in the ascidian Botryllus schlosseri. Mol Rep Dev
Kassmer, S.H., Rodriguez, D., Langenbacher, A.D., Bui C., and De Tomaso, A.W. (2015) Migration of germline progenitor cells is directed by sphingosine-1-phosphate signaling in a basal chordate. Nature Communications 12:8565
Taketa, D.A., Nydam, M.L., Langenbacher, A.D.,Rodriguez, D., Sanders, E., and De Tomaso A.W. (2015) Molecular evolution and in vitro characterization of Botryllus histocompatibility factor. Immunogenetics 67:605-23.
Rodriguez, D., Sanders E.N., Farell, K., Langenbacher, A.D., Taketa, D.A., Hopper, M.R., Kennedy, M., Gracey, A.Y., De Tomaso, A.W. (2014) Analysis of the basal chordate Botryllus schlosseri reveals a set of genes associated with fertility. BMC Genomics 15:1183.
Braden, B.P., Taketa D.A., Pierce, J.D., Kassmer, S., Lewis, D.D. and De Tomaso, A.W. (2014) Vascular regeneration in a basal chordate is due to the presence of immobile, bi-functional cells. PLoS One e95460