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Christopher S. Hayes

Associate Professor

Contact Information

Phone: (805) 893-2028
Email: chayes@lifesci.ucsb.edu
Office: 3105 LSB

Address

Molecular, Cellular, and Developmental Biology
University of California, Santa Barbara
Santa Barbara, CA 93106-9625

Bio

Dr. Hayes received his B.A. (Biology) and M.S. (Applied Immunology) degrees from the University of Southern Maine, and his Ph.D. in Molecular Biology & Biochemistry from the University of Connecticut School of Medicine. He was a Walter Winchell-Damon Runyon Cancer Research Fund postdoctoral fellow at the Massachusetts Institute of Technology and joined the MCDB faculty in 2004.

Research

The Hayes lab studies the molecular biology and biochemistry of prokaryotic ribonucleases that regulate gene expression and cell growth. The two main research interests revolve around the mechanisms of ribosome rescue and the function of cytotoxic nucleases encoded by contact-dependent growth inhibition (CDI) systems. Bacterial ribosome rescue is mediated by the transfer-messenger RNA (tmRNA) and alternative ribosome rescue (ArfA) quality control systems, which co-operate to maintain active ribosome pools. CDI systems are widely distributed throughout α, β and γ-proteobacteria and function in growth competition. Many of these systems deploy toxic nucleases that are delivered into the cytosol of susceptible target bacteria. We are interested in the mechanisms of nuclease delivery, activation and immunity during CDI.

Selected Publications

  • Ruhe, Z. C., Low, D. A. and C. S. Hayes (2013) Bacterial contact-dependent growth inhibition (CDI). Trends in Microbiology 21:230-237
  • Webb, J. S., Nikolakakis, K. C., Willett, J. L. E., Aoki, S. K., Hayes, C. S. and D. A. Low (2013) Delivery of CdiA nuclease toxins into target cells during contact-dependent growth inhibition (CDI). PLoS ONE 8:e57609
  • Koskiniemi, S., Lamoureux, J. G., Nikolakakis, K. C., t’Kint de Roodenbeke, C., Kaplan, M. D., Low, D. A. and C. S. Hayes (2013) Rhs proteins from diverse bacteria mediate intercellular competition. Proceedings of the National Academy of Sciences U.S.A. 110:7032-7037
  • Morse, R., Nikolakakis, K. C., Willett, J. L. E., Gerrick, E., Low, D., A., Hayes, C. S. and C. W. Goulding (2012) Structural basis for toxicity and immunity in contact-dependent growth inhibition (CDI) systems. Proceedings of the National Academy of Sciences U.S.A. 109:21480-21485
  • Schaub, R. E., Poole, S. J., Garza-Sánchez, F., Benbow, S. and C. S. Hayes (2012) Proteobacterial ArfA peptides are synthesized from non-stop messenger RNAs. Journal of Biological Chemistry 287:29765-29775
  • Nikolakakis, K., Amber, S., Diner, E. J., Aoki, S. K., Poole, S. J., Tuanyok, A., Keim, P., Peacock, S., Hayes, C. S. and D. A. Low (2012) The toxin/immunity network of Burkholderia pseudomallei contact-dependent growth inhibition (CDI) systems. Molecular Microbiology 84:516-529
  • Janssen, B. D., Diner, E. J. and C. S. Hayes (2012) Analysis of aminoacyl- and peptidyl-tRNAs by gel electrophoresis. Methods in Molecular Biology 905:291-309.
  • Diner, E. J., Beck, C. M., Webb, J. S., Low, D. A., and C. S. Hayes (2012) Identification of a target cell permissive factor required for contact-dependent growth inhibition (CDI). Genes and Development 26:515-525
  • Holberger, L. E., Garza-Sánchez, F., Lamoureux, J., Low, D. A., and C. S. Hayes (2012) A novel family of toxin/antitoxin proteins in Bacillus species. FEBS Letters 586:132-136
  • Janssen, B. D., and C. S. Hayes (2012) The tmRNA ribosome-rescue system. Advances in Protein Chemistry and Structural Biology 86:151-191
  • Seidman, J. S., Janssen, B. D., and C. S. Hayes (2011) Alternative fates of paused ribosomes during translation termination. Journal of Biological Chemistry 286:31105-31112
  • Poole, S. J., Diner, E. J., Aoki, S. K., Braaten, B. A., Low, D. A., and C. S. Hayes (2011) Identification of functional toxin/immunity genes linked to contact-dependent growth inhibition (CDI) and rearrangement hotspot (Rhs) systems. PLoS Genetics 7:e1002217
  • Diner, E. J., Garza-Sánchez, F., and C. S. Hayes (2011) Genome engineering using targeted oligonucleotide libraries and functional selection. Methods in Molecular Biology 765:71-82
  • Aoki, S. K., Poole, S. J., Hayes, C. S., and D. A. Low (2011) Toxin on a stick: modular CDI toxin delivery systems play roles in bacterial competition. Virulence 2:356-359
  • Garza-Sánchez, F., Schaub, R. E., Janssen, B. D., and C. S. Hayes (2011) tmRNA regulates synthesis of the ArfA ribosome rescue factor. Molecular Microbiology 80:1204-1219
  • Schaub, R. E., and C. S. Hayes (2011) Deletion of the RluD pseudouridine synthase promotes SsrA peptide tagging of ribosomal protein S7. Molecular Microbiology 79:331-341
  • Ruhe, Z. C., and C. S. Hayes (2010) The N-terminus of GalE induces tmRNA activity in Escherichia coli. PLoS ONE 5:e15207
  • Aoki, S. K., Diner, E. J., t'Kint de Roodenbeke, C., Burgess, B. R., Poole, S. J., Braaten, B. A., Jones, A. M., Webb, J. S., Hayes, C. S., Cotter, P. A., and D. A. Low (2010) A widespread family of polymorphic contact-dependent toxin delivery systems in bacteria. Nature 468:439-442
  • Hayes, C. S., Aoki, S. K., and D. A. Low (2010) Bacterial contact-dependent delivery systems. Annual Review of Genetics 44:71-90
  • Hayes, C. S., and K. C. Keiler (2010) Beyond ribosome rescue: tmRNA and co-translational processes. FEBS Letters 584:413-419
All Publications